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  1. Sakata, N., Fujikawa, T., Uke, A., Ishiga, T., Ichinose, Y. and Ishiga, Y. (2023) HexR transcription factor contributes to Pseudomonas cannabina pv. alisalensis virulence by coordinating type three secretion system genes. Microorganisms.

  2. Nguyen, V. T., Sakata, N., Ishiga, T., Usuki, G., Hashimoto, Y. and Ishiga, Y. (2023) Vt35 antitoxin plays a central regulatory role in virulence of Pseudomonas savastanoi pv. glycinea on soybean. J. Gen. Plant Pathol.

  3. Sakata, N. and Ishiga, Y. (2023) Prevention of stomatal entry as a strategy for plant disease control against foliar pathogenic Pseudomonas species. Plants 12: 590

  4. Ishiga, T., Sakata, N., Usuki G., Nguyen, V. T., Gomi K. and Ishiga, Y. (2023) Large-scale transposon mutagenesis reveals type III secretion effector HopR1 is a major virulence factor in Pseudomonas syringae pv. actinidiae. Plants 12: 141

  5. Sakata, N., Ino, T., Ishiga, T. and Ishiga, Y. (2023) Controlling stomatal aperture, a potential strategy for managing plant bacterial disease. Plant Science 327

  6. Sakata, N., Shiraishi, N., Saito, H., Komoto, H., Ishiga, T., Usuki, G., Yamashita, Y. and Ishiga, Y. (2023) Covering cabbage leaves with cellulose nanofiber confers resistance against Pseudomonas cannabina pv. alisalensis. J. Gen. Plant Pathol. 89：53–60

  7. Sakata, N., Masuo, S., Nagayoshi, R., Ishiga, T., and Ishiga. Y. (2022) Translocation of acibenzolar, an active metabolic substance of acibenzolar-S-methyl, to distal leaves in cabbage and Japanese radish. J. Gen. Plant Pathol. 88：392–398

  8. Sakata, N., Haraguchi, T., Masuo, S., Ishiga, T. and Ishiga, Y. (2022) Pseudomonas cannabina pv. alisalensis virulence factors are involved in resistance to plant-derived antimicrobials during infection. Plants. 11:1742

  9. Matsuo, H., Ishiga, Y., Kubo, Y. and Yoshioka, Y. (2022) Colletotrichum orbiculare strains distributed in Japan: race identification and evaluation of virulence to cucurbits. Breeding Science.

  10. Fukudome, A., Ishiga, Y., Nagashima, Y., Davidson, K. H., Chou, H. A., Mysore, K. S. and Koiwa, H. (2022) Functional diversity of RNA polymerase II CTD phosphatase isoforms produced in the superexpression platform. Plant Science. 321. https://doi.org/10.1016/j.plantsci.2022.111309.

  11. Saito, H., Sakata, N., Ishiga, T. and Ishiga, Y. (2022) Efficacy of RNA-spray-induced silencing of Phakopsora pachyrhizi chitin synthase genes to control soybean rust. J. Gen. Plant Pathol. 88: 203–206. https://doi.org/10.1007/s10327-022-01061-w.

  12. Nguyen, V. T., Sakata, N., Usuki, G., Ishiga, T., Hashimoto, Y. and Ishiga, Y. (2021) Multiple virulence factors regulated by AlgU contribute to the pathogenicity of Pseudomonas savastanoi pv. glycinea in soybean. PeerJ 9:e12405 https://doi.org/10.7717/peerj.12405.

  13. Saito, H., Yamashita, Y., Sakata, N., Ishiga, T., Shiraishi, N., Usuki, G., Nguyen, V. T., Yamamura E. and Ishiga, Y. (2021) Covering soybean leaves with cellulose nanofiber changes leaf surface hydrophobicity and confers resistance against Phakopsora pachyrhizi. Frontiers in Plant Science. doi: 10.3389/fpls.2021.726565.

  14. Sakata, N., Ishiga, T., Aoyagi, T. and Ishiga, Y. (2021) Acibenzolar-S-methyl efficacy against bacterial brown stripe caused by Acidovorax avenae subsp. avenae in creeping bentgrass. J. Gen. Plant Pathol. 87:387–393.

  15. Sakata, N., Ishiga, T., Masuo, S., Hashimoto, Y. and Ishiga, Y. (2021) Coronatine contributes to Pseudomonas cannabina pv. alisalensis virulence by overcoming both stomatal and apoplastic defenses in dicot and monocot plants. Mol Plant Microbe Interact.34:746-757.

  16. Sakata, N., Ishiga, T., and Ishiga, Y. (2021) Pseudomonas cannabina pv. alisalensis TrpA is required for virulence in multiple host plants. Frontiers in Microbiology. 12: 659734. https://doi.org/10.3389/fmicb.2021.659734.

  17. Ishiga, T., Sakata, N., Ugajin., T. and Ishiga, Y. (2021) Acibenzolar-S-methyl and probenazole control bacterial blight disease of cabbage by activating stomatal-based defense in different timing manners. J. Gen. Plant Pathol. 87, 30–34.

  18. Sakata, N., Ishiga, T., Taniguchi, S. and Ishiga, Y. (2020) Acibenzolar-S-methyl activates stomatal-based defense systemically in Japanese radish. Frontiers in Plant Science. 11, 1670.

  19. Hirose, K., Ishiga, Y. and Fujikawa, T. (2020) Phytotoxin synthesis genes and type III effector genes of Pseudomonas syringae pv. actinidiae biovar 6 are regulated by culture conditions. PeerJ 8:e9697. doi: 10.7717/peerj.9697. eCollection 2020.

  20. Ishiga, T., Sakata, N., Nguyen, V. T. and Ishiga, Y. (2020) Flood-inoculation of seedlings on culture medium to study interactions between Pseudomonas syringae pv. actinidiae and kiwifruit. J. Gen. Plant Pathol. 86, 257–265

  21. Ishiga, T., Iida, Y., Sakata, N., Ugajin, T., Hirata, T., Taniguchi, S., Hayashi, K. and Ishiga, Y. (2020) Acibenzolar-S-methyl activates stomatal-based defense against Pseudomonas cannabina pv. alisalensis in cabbage. J. Gen. Plant Pathol. 86:48-54

  22. Sakata, N., Ishiga, T., Saito, H., Nguyen, V. T. and Ishiga, Y. (2019) Transposon mutagenesis reveals Pseudomonas cannabina pv. alisalensis optimizes its virulence factors for pathogenicity on different hosts. PeerJ. 20;7:e7698.

  23. Inoue, T., Okane, I., Ishiga, Y., Degawa, Y., Hosoya, T., Yamaoka, Y. (2019) The life cycle of s on Manchurian ash, Fraxinus mandshurica, in Japan. Mycoscience. . 60:89-94.

  24. Hossain, Z., ., Ishiga, Y., Yamaoka, N., Ogiso-Tanaka, E. and Yamaoka, Y. (2018) Soybean leaves transcriptomic data dissects the phenylpropanoid pathway genes as a defence response against Phakopsora pachyrhizi. Plant Physiology and Biochemistry.132:424-433.

  25. Ishiga, T., Ishiga, Y., Betsuyaku, S. and Nomura, N. (2017) AlgU contributes to the virulence of Pseudomonas syringae pv. tomato DC3000 by regulating phytotoxin coronatine production. J. Gen. Plant Pathol. 81:189-201.

  26. Gill, U.S., Uppalapati, S.R., Gallego-Giraldo, L., Ishiga, Y., Dixon, R.A. and Mysore, K.S. 2017. Metabolic flux towards the (iso)flavonoid pathway in lignin modified alfalfa lines induces resistance against Fusarium oxysporum f. sp. medicaginis. Plant, Cell & Environment. In press.

  27. Ishiga, Y., Watanabe, M., Ishiga, T., Tohge, T., Matsuura, T., Ikeda, Y., Hoefgen, R., Fernie, A.R. and Mysore, K.S. (2017) The SAL-PAP chloroplast retrograde pathway contributes to plant immunity by regulating glucosinolate pathway and phytohormone signaling. Mol. Plant-Microbe Interact. 30:829-841.

  28. Lee, S., Muthappa, S., Kang, K., Rojas, C., Tang, Y., Oh, S., Choudhury, S.R. Lee, H.K., Ishiga, Y., Allen, R., Pandey, S. and Mysore, K.S. (2017) The small GTPase, nucleolar GTP-binding protein 1 (NOG1), has a novel role in plant innate immunity. Scientific Reports. 7:9260.

  29. Ishiga, Y., Ishiga, T., Ichinose, Y. and Mysore, K.S. (2016) Pseudomonas syringae Flood-inoculation Method in Arabidopsis. Bio-protocol. 7(2): e2106.

  30. Miwa, E., Okane, I., Ishiga, Y., Sugawara, K. and Yamaoka, Y. (2016) Confirmation of taxonomic status of an species on in Japan. Mycoscience. 58:147-153.

  31. Ishiga, Y., Ishiga, T., Ikeda, Y., Matsuura, T. and Mysore, K.S. (2016) NADPH-dependent thioredoxin reductase C plays a role in nonhost disease resistance against Pseudomonas syringae pathogens by regulating chloroplast-generated reactive oxygen species. PeerJ. 4:e1938.

  32. Toyoda, K., Yao, S., Takagi, M., Uchioki, M., Miki, M., Tanaka, K., Suzuki, T., Amano, M., Kiba, A., Kato, T., Takahashi, H., Ishiga, Y., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y. and Shiraishi, T. (2016) Plant cell wall as a site for molecular contacts in fungal pathogenesis. Physiological and Molecular Plant Pathology. 95：44-49.

  33. Takagi, H., Ishiga, Y., Watanabe, S., Konishi, T., Egusa, M., Akiyoshi, N., Matsuura, T., Mori, I., Hirayama, T., Kaminaka, H., Shimada, H. and Sakamoto, A. (2016) Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner. Journal Of Experimental Botany. 678:2519-32.

  34. Ishiga, Y. and Ichinose, Y. (2016) Pseudomonas syringae pv. tomato OxyR is required for virulence in tomato and Arabidopsis. Mol. Plant-Microbe Interact. 29:119-31.

  35. Ishiga, Y., Uppalapati, S.R., Gill, U.S., Huhman, D., Tang, Y. and Mysore, K.S. (2015) Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust. Scientific Reports 12;5:1306.

  36. Lee, S., Rojas, C.M., Ishiga, Y., Pandey, S. and Mysore, K.S. (2013) Arabidopsis heterotrimeric G-proteins play a critical role in host and nonhost resistance against Pseudomonas syringae pathogens. PLoS ONE 8(12): e82445. doi:10.1371/journal.pone.0082445.

  37. Ishiga, Y., Uppalapati, S.R. and Mysore, K.S. (2013) Expression analysis reveals a role for hydrophobic or epicuticular wax signals in pre-penetration structure formation of Phakopsora pachyrhizi. Plant Signal. Behav.

  38. Ishiga, Y., Ishiga, T., Uppalapati, S.R. and Mysore, K.S. (2013) Jasmonate ZIM-domain (JAZ) protein regulates host and nonhost pathogen-induced cell death in tomato and Nicotiana benthamiana. PLoS ONE 8: e75728. doi:10.1371/journal.pone.0075728.

  39. Venkategowda, R., Muthappa, S.K, Ishiga, Y., Kaundal, A., Udayakumar, M. and Mysore, K.S. (2013) Drought stress acclimation imparts tolerance to Sclerotinia sclerotiorum and Pseudomonas syringae in Nicotiana benthamiana. Int. J. Mol. Sci. 14: 9497-9513. doi: 10.3390/ijms14059497.

  40. Lee, S., Ishiga, Y., Clermont, K. and Mysore, K.S. (2013) Coronatine inhibits stomatal closure and delays hypersensitive response cell death induced by nonhost bacterial pathogens. PeerJ 1: e34. doi: 10.7717/peerj.34.

  41. Uppalapati, S.R., Serba, D.D., Ishiga, Y., Szabo, L.J., Mittal, S., Bhandari, H.S., Bouton, J.H., Mysore, K.S. and Saha, M.C. (2013) Characterization of the rust fungus, Puccinia emaculata, and evaluation of genetic variability for rust resistance in switchgrass populations. BioEnergy Research 6: 458-468. doi: 10.1007/s12155-012-9263-6.

  42. Uppalapati, S.R., Ishiga, Y., Doraiswamy, V., Bedair, M., Mittal, S., Chen, J., Nakashima, J., Tang, Y., Tadege, M., Ratet, P., Chen, R., Schultheiss, H. and Mysore K.S. (2012) Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens. The Plant Cell 24: 353-370. doi: 10.1105/tpc.111.093104. Co-first author

  43. Ishiga, Y., Ishiga, T. Wangdi, T., Mysore, K.S. and Uppalapati, S.R. (2012) NTRC and chloroplast-generated reactive oxygen species regulate Pseudomonas syringae pv. tomato disease development in tomato and Arabidopsis. Mol. Plant-Microbe Interact. 25: 294-306. doi: 10.1094/MPMI-05-11-0130.

  44. Ishiga, Y., Ishiga, T., Uppalapati, S.R. and Mysore, K.S. (2011) Arabidopsis seedling-flood inoculation technique: A rapid and reliable assay for studying plant-bacterial interactions. Plant Methods 7:32. doi: 10.1186/1746-4811-7-32.

  45. Ishiga, Y., Uppalapati, S.R., Ishiga, T. and Mysore, K.S. (2011) Involvement of SGT1 in COR-mediated signal transduction pathway leading to disease symptom development. Plant Signal. Behav. 6: 1072-1073. 10.4161/psb.6.7.15652.

  46. Uppalapati, S.R., Ishiga, Y., Ryu, C.M., Ishiga, T., Wang, K., Noël, L.D., Parker, J.E. and Mysore K.S. (2011) SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis. New Phytol. 189: 83-93. doi: 10.1111/j.1469-8137.2010.03470.x. Co-first author

  47. Ishiga, Y., Uppalapati, S.R., Ishiga, T. and Bender, C.L. (2010) Exogenous coronatine, but not coronafacic acid or methyl jasmonate, restores the disease phenotype of a coronatine defective mutant of Pseudomonas syringae pv. tomato on tomato seedlings. J. Gen. Plant Pathol. 76: 188-195. doi: 10.1007/s10327-010-0228-3.

  48. Yakushiji, S., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2009) Bacterial DNA activates immunity in Arabidopsis thaliana. J. Gen. Plant Pathol. 75: 227-234. doi: 10.1007/s10327-009-0162-4.

  49. Ishiga, Y., Uppalapati, S.R., Ishiga, T. Elavarthi, S., Martin, B. and Bender, C.L. (2009) Involvement of coronatine-inducible reactive oxygen species in disease-associated necrotic cell death during bacterial speck of tomato. Plant Signal. Behav. 4: 237-239. 10.4161/psb.4.3.7915.

  50. Ishiga, Y., Uppalapati, S.R., Ishiga, T., Elavarthi, S., Martin, B. and Bender, C.L. (2009) The phytotoxin coronatine induces light-dependent reactive oxygen species in tomato seedlings. New Phytol. 181: 147-160. doi: 10.1111/j.1469-8137.2008.02639.x.

  51. Uppalapati, S.R., Ishiga, Y., Wangdi, T., Ishiga, T. and Bender, C.L. (2008) Pathogenicity of Pseudomonas syringae pv. tomato on tomato seedlings: phenotypic and gene expression analyses of the virulence function of coronatine. Mol. Plant-Microbe Interact. 21: 383-395. doi: 10.1094/MPMI-21-4-0383. Co-first author

  52. Higashi, K., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T and Ichinose, Y. (2008) Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana. Mol. Genet. Genomics 279: 303-312. doi: 10.1007/s00438-007-0315-0.

  53. Uppalapati, S.R., Ishiga, Y., Wangdi, T., Kunkel, B.N., Anand, A., Mysore, K.S. and Bender. C. L. (2007) The phytotoxin coronatine is required for pathogen fitness and suppression of salicylic acid accumulation in tomato inoculated with Pseudomonas syringae pv. tomato DC3000. Mol. Plant-Microbe Interact. 20: 955-965. doi:10.1094 / MPMI -20-8-0955.

  54. Naito, K., Ishiga, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2007) N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana. J. Gen. Plant Pathol. 73: 281-285. doi: 10.1007/s10327-007-0017-9.

  55. Ishiga, Y., Takeuchi, K., Taguchi, F., Inagaki, Y., Toyoda, K., Ichinose, Y. (2005) Defense responses of Arabidopsis thaliana inoculated with Pseudomonas syringae pv. tabaci wild type and defective mutants for flagellin (∆fliC) and flagellin-glycosylation (∆orf1). J. Gen. Plant Pathol. 71: 327-307. doi: 10.1007/s10327-005-0201-8.

  56. Uppalapati, S. R., Toyoda, K., Ishiga, Y., Ichinose, Y. and Shiraishi, T. Differential regulation of MBP kinases by a glycoprotein elicitor and a polypeptide suppressor from Mycosphaerella pinodes in pea. Physiol. Mol. Plant Pathol. 64: 17-25. doi: 10.1016/j.pmpp.2004.05.003.

  57. Matsui, H., Nakamura, G., Ishiga, Y., Toshima, H., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2004) Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products. Mol. Genet. Genomics 271: 1-10. doi: 10.1007/s00438-003-0948-6.

  58. Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y. (2003) Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens. J. Gen. Plant Pathol. 69: 351-357. doi: 10.1007/s10327-003-0061-z.

  59. Ishiga, Y., Funato, A., Tachiki, T., Toyoda, K., Shiraishi, T., Yamada, T. and Ichinose, Y. (2002) Expression of the 12-Oxophytodienoic acid 10, 11-reductase gene in the compatible interaction between pea and fungal pathogen. Plant Cell Physiol. 43: 1210-1220. doi: 10.1093/pcp/pcf144.

  60. Ichinose, Y., Hisayasu, Y., Sanematsu, S., Ishiga, Y., Seki, H., Toyoda, K., Shiraishi, T., Yamada, T. (2001) Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity related hsr203J. Plant Sci. 160: 997-1006. doi: 10.1016/S0168-9452(01)00343-0.

 

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